mouse anti tfap2α Search Results


96
Developmental Studies Hybridoma Bank tfap2α
Tfap2α, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tfap2α/product/Developmental Studies Hybridoma Bank
Average 96 stars, based on 1 article reviews
tfap2α - by Bioz Stars, 2026-04
96/100 stars
  Buy from Supplier

95
Santa Cruz Biotechnology anti tfap2α antibodies
(A) In vitro eTw-5 activity in HEK293T cells following co-transfection with LMX1B or <t>TFAP2</t> (TFAP2α and/or TFAP2γ) expression vectors. Luciferase activity was measured for the eTw-5 sequence and for deleted LMX1B (ΔLMX1B), TFAP2 (ΔTFAP2) or both LMX1B and TFAP2 (ΔLMX1B\ΔTFAP2) binding sites from eTw-5 sequence. (B) In vitro eTw-6 activity in HEK293T cells following co-transfection with LMX1B or TFAP2 (TFAP2α and/or TFAP2γ) expression vectors. Luciferase activity was measured for the eTw-6 sequence and deleted LMX1B (ΔLMX1B), TFAP2 (ΔTFAP2) or both LMX1B and TFAP2 (ΔLMX1B\ΔTFAP2) binding sites from eTw-6 sequence. (C) In vitro eTw-7 activity in HEK293T cells following co-transfection with LMX1B expression vector. Luciferase activity was measured for the eTw-7 sequence and for LMX1B-deleted eTw-7 (ΔLMX1B). Relative luciferase expression results after normalization to Renilla activity are presented and represent the means ± standard deviation of three independent experiments (P-value<0.05, Student’s t-test). (D) ChIP-qPCR analysis of eTw5-7 with anti-TFAP2α antibodies in a mouse E11.5 limb bud. Fold enrichment is presented as a fraction of input. The error bars represent the SD from two technical replicates of a representative experiment. (E) A schematic representation of minimal enhancer sequences with the location of the predicted binding site for TFAP2 (red bar) and LMX1B (green bar) shown.
Anti Tfap2α Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti tfap2α antibodies/product/Santa Cruz Biotechnology
Average 95 stars, based on 1 article reviews
anti tfap2α antibodies - by Bioz Stars, 2026-04
95/100 stars
  Buy from Supplier

91
Developmental Studies Hybridoma Bank mouse anti tfap2α
(A) In vitro eTw-5 activity in HEK293T cells following co-transfection with LMX1B or <t>TFAP2</t> (TFAP2α and/or TFAP2γ) expression vectors. Luciferase activity was measured for the eTw-5 sequence and for deleted LMX1B (ΔLMX1B), TFAP2 (ΔTFAP2) or both LMX1B and TFAP2 (ΔLMX1B\ΔTFAP2) binding sites from eTw-5 sequence. (B) In vitro eTw-6 activity in HEK293T cells following co-transfection with LMX1B or TFAP2 (TFAP2α and/or TFAP2γ) expression vectors. Luciferase activity was measured for the eTw-6 sequence and deleted LMX1B (ΔLMX1B), TFAP2 (ΔTFAP2) or both LMX1B and TFAP2 (ΔLMX1B\ΔTFAP2) binding sites from eTw-6 sequence. (C) In vitro eTw-7 activity in HEK293T cells following co-transfection with LMX1B expression vector. Luciferase activity was measured for the eTw-7 sequence and for LMX1B-deleted eTw-7 (ΔLMX1B). Relative luciferase expression results after normalization to Renilla activity are presented and represent the means ± standard deviation of three independent experiments (P-value<0.05, Student’s t-test). (D) ChIP-qPCR analysis of eTw5-7 with anti-TFAP2α antibodies in a mouse E11.5 limb bud. Fold enrichment is presented as a fraction of input. The error bars represent the SD from two technical replicates of a representative experiment. (E) A schematic representation of minimal enhancer sequences with the location of the predicted binding site for TFAP2 (red bar) and LMX1B (green bar) shown.
Mouse Anti Tfap2α, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti tfap2α/product/Developmental Studies Hybridoma Bank
Average 91 stars, based on 1 article reviews
mouse anti tfap2α - by Bioz Stars, 2026-04
91/100 stars
  Buy from Supplier

99
Developmental Studies Hybridoma Bank mouse anti n cadherin
(A) In vitro eTw-5 activity in HEK293T cells following co-transfection with LMX1B or <t>TFAP2</t> (TFAP2α and/or TFAP2γ) expression vectors. Luciferase activity was measured for the eTw-5 sequence and for deleted LMX1B (ΔLMX1B), TFAP2 (ΔTFAP2) or both LMX1B and TFAP2 (ΔLMX1B\ΔTFAP2) binding sites from eTw-5 sequence. (B) In vitro eTw-6 activity in HEK293T cells following co-transfection with LMX1B or TFAP2 (TFAP2α and/or TFAP2γ) expression vectors. Luciferase activity was measured for the eTw-6 sequence and deleted LMX1B (ΔLMX1B), TFAP2 (ΔTFAP2) or both LMX1B and TFAP2 (ΔLMX1B\ΔTFAP2) binding sites from eTw-6 sequence. (C) In vitro eTw-7 activity in HEK293T cells following co-transfection with LMX1B expression vector. Luciferase activity was measured for the eTw-7 sequence and for LMX1B-deleted eTw-7 (ΔLMX1B). Relative luciferase expression results after normalization to Renilla activity are presented and represent the means ± standard deviation of three independent experiments (P-value<0.05, Student’s t-test). (D) ChIP-qPCR analysis of eTw5-7 with anti-TFAP2α antibodies in a mouse E11.5 limb bud. Fold enrichment is presented as a fraction of input. The error bars represent the SD from two technical replicates of a representative experiment. (E) A schematic representation of minimal enhancer sequences with the location of the predicted binding site for TFAP2 (red bar) and LMX1B (green bar) shown.
Mouse Anti N Cadherin, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti n cadherin/product/Developmental Studies Hybridoma Bank
Average 99 stars, based on 1 article reviews
mouse anti n cadherin - by Bioz Stars, 2026-04
99/100 stars
  Buy from Supplier

95
Developmental Studies Hybridoma Bank mouse anti laminin
(A) In vitro eTw-5 activity in HEK293T cells following co-transfection with LMX1B or <t>TFAP2</t> (TFAP2α and/or TFAP2γ) expression vectors. Luciferase activity was measured for the eTw-5 sequence and for deleted LMX1B (ΔLMX1B), TFAP2 (ΔTFAP2) or both LMX1B and TFAP2 (ΔLMX1B\ΔTFAP2) binding sites from eTw-5 sequence. (B) In vitro eTw-6 activity in HEK293T cells following co-transfection with LMX1B or TFAP2 (TFAP2α and/or TFAP2γ) expression vectors. Luciferase activity was measured for the eTw-6 sequence and deleted LMX1B (ΔLMX1B), TFAP2 (ΔTFAP2) or both LMX1B and TFAP2 (ΔLMX1B\ΔTFAP2) binding sites from eTw-6 sequence. (C) In vitro eTw-7 activity in HEK293T cells following co-transfection with LMX1B expression vector. Luciferase activity was measured for the eTw-7 sequence and for LMX1B-deleted eTw-7 (ΔLMX1B). Relative luciferase expression results after normalization to Renilla activity are presented and represent the means ± standard deviation of three independent experiments (P-value<0.05, Student’s t-test). (D) ChIP-qPCR analysis of eTw5-7 with anti-TFAP2α antibodies in a mouse E11.5 limb bud. Fold enrichment is presented as a fraction of input. The error bars represent the SD from two technical replicates of a representative experiment. (E) A schematic representation of minimal enhancer sequences with the location of the predicted binding site for TFAP2 (red bar) and LMX1B (green bar) shown.
Mouse Anti Laminin, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti laminin/product/Developmental Studies Hybridoma Bank
Average 95 stars, based on 1 article reviews
mouse anti laminin - by Bioz Stars, 2026-04
95/100 stars
  Buy from Supplier

94
Developmental Studies Hybridoma Bank mouse anti sox9
(A) In vitro eTw-5 activity in HEK293T cells following co-transfection with LMX1B or <t>TFAP2</t> (TFAP2α and/or TFAP2γ) expression vectors. Luciferase activity was measured for the eTw-5 sequence and for deleted LMX1B (ΔLMX1B), TFAP2 (ΔTFAP2) or both LMX1B and TFAP2 (ΔLMX1B\ΔTFAP2) binding sites from eTw-5 sequence. (B) In vitro eTw-6 activity in HEK293T cells following co-transfection with LMX1B or TFAP2 (TFAP2α and/or TFAP2γ) expression vectors. Luciferase activity was measured for the eTw-6 sequence and deleted LMX1B (ΔLMX1B), TFAP2 (ΔTFAP2) or both LMX1B and TFAP2 (ΔLMX1B\ΔTFAP2) binding sites from eTw-6 sequence. (C) In vitro eTw-7 activity in HEK293T cells following co-transfection with LMX1B expression vector. Luciferase activity was measured for the eTw-7 sequence and for LMX1B-deleted eTw-7 (ΔLMX1B). Relative luciferase expression results after normalization to Renilla activity are presented and represent the means ± standard deviation of three independent experiments (P-value<0.05, Student’s t-test). (D) ChIP-qPCR analysis of eTw5-7 with anti-TFAP2α antibodies in a mouse E11.5 limb bud. Fold enrichment is presented as a fraction of input. The error bars represent the SD from two technical replicates of a representative experiment. (E) A schematic representation of minimal enhancer sequences with the location of the predicted binding site for TFAP2 (red bar) and LMX1B (green bar) shown.
Mouse Anti Sox9, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti sox9/product/Developmental Studies Hybridoma Bank
Average 94 stars, based on 1 article reviews
mouse anti sox9 - by Bioz Stars, 2026-04
94/100 stars
  Buy from Supplier

90
Millipore mouse anti-β-catenin
(A) In vitro eTw-5 activity in HEK293T cells following co-transfection with LMX1B or <t>TFAP2</t> (TFAP2α and/or TFAP2γ) expression vectors. Luciferase activity was measured for the eTw-5 sequence and for deleted LMX1B (ΔLMX1B), TFAP2 (ΔTFAP2) or both LMX1B and TFAP2 (ΔLMX1B\ΔTFAP2) binding sites from eTw-5 sequence. (B) In vitro eTw-6 activity in HEK293T cells following co-transfection with LMX1B or TFAP2 (TFAP2α and/or TFAP2γ) expression vectors. Luciferase activity was measured for the eTw-6 sequence and deleted LMX1B (ΔLMX1B), TFAP2 (ΔTFAP2) or both LMX1B and TFAP2 (ΔLMX1B\ΔTFAP2) binding sites from eTw-6 sequence. (C) In vitro eTw-7 activity in HEK293T cells following co-transfection with LMX1B expression vector. Luciferase activity was measured for the eTw-7 sequence and for LMX1B-deleted eTw-7 (ΔLMX1B). Relative luciferase expression results after normalization to Renilla activity are presented and represent the means ± standard deviation of three independent experiments (P-value<0.05, Student’s t-test). (D) ChIP-qPCR analysis of eTw5-7 with anti-TFAP2α antibodies in a mouse E11.5 limb bud. Fold enrichment is presented as a fraction of input. The error bars represent the SD from two technical replicates of a representative experiment. (E) A schematic representation of minimal enhancer sequences with the location of the predicted binding site for TFAP2 (red bar) and LMX1B (green bar) shown.
Mouse Anti β Catenin, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-β-catenin/product/Millipore
Average 90 stars, based on 1 article reviews
mouse anti-β-catenin - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

93
Developmental Studies Hybridoma Bank mouse anti hnk1
(A) In vitro eTw-5 activity in HEK293T cells following co-transfection with LMX1B or <t>TFAP2</t> (TFAP2α and/or TFAP2γ) expression vectors. Luciferase activity was measured for the eTw-5 sequence and for deleted LMX1B (ΔLMX1B), TFAP2 (ΔTFAP2) or both LMX1B and TFAP2 (ΔLMX1B\ΔTFAP2) binding sites from eTw-5 sequence. (B) In vitro eTw-6 activity in HEK293T cells following co-transfection with LMX1B or TFAP2 (TFAP2α and/or TFAP2γ) expression vectors. Luciferase activity was measured for the eTw-6 sequence and deleted LMX1B (ΔLMX1B), TFAP2 (ΔTFAP2) or both LMX1B and TFAP2 (ΔLMX1B\ΔTFAP2) binding sites from eTw-6 sequence. (C) In vitro eTw-7 activity in HEK293T cells following co-transfection with LMX1B expression vector. Luciferase activity was measured for the eTw-7 sequence and for LMX1B-deleted eTw-7 (ΔLMX1B). Relative luciferase expression results after normalization to Renilla activity are presented and represent the means ± standard deviation of three independent experiments (P-value<0.05, Student’s t-test). (D) ChIP-qPCR analysis of eTw5-7 with anti-TFAP2α antibodies in a mouse E11.5 limb bud. Fold enrichment is presented as a fraction of input. The error bars represent the SD from two technical replicates of a representative experiment. (E) A schematic representation of minimal enhancer sequences with the location of the predicted binding site for TFAP2 (red bar) and LMX1B (green bar) shown.
Mouse Anti Hnk1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti hnk1/product/Developmental Studies Hybridoma Bank
Average 93 stars, based on 1 article reviews
mouse anti hnk1 - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier

90
Millipore rabbit anti-sox9
(A) In vitro eTw-5 activity in HEK293T cells following co-transfection with LMX1B or <t>TFAP2</t> (TFAP2α and/or TFAP2γ) expression vectors. Luciferase activity was measured for the eTw-5 sequence and for deleted LMX1B (ΔLMX1B), TFAP2 (ΔTFAP2) or both LMX1B and TFAP2 (ΔLMX1B\ΔTFAP2) binding sites from eTw-5 sequence. (B) In vitro eTw-6 activity in HEK293T cells following co-transfection with LMX1B or TFAP2 (TFAP2α and/or TFAP2γ) expression vectors. Luciferase activity was measured for the eTw-6 sequence and deleted LMX1B (ΔLMX1B), TFAP2 (ΔTFAP2) or both LMX1B and TFAP2 (ΔLMX1B\ΔTFAP2) binding sites from eTw-6 sequence. (C) In vitro eTw-7 activity in HEK293T cells following co-transfection with LMX1B expression vector. Luciferase activity was measured for the eTw-7 sequence and for LMX1B-deleted eTw-7 (ΔLMX1B). Relative luciferase expression results after normalization to Renilla activity are presented and represent the means ± standard deviation of three independent experiments (P-value<0.05, Student’s t-test). (D) ChIP-qPCR analysis of eTw5-7 with anti-TFAP2α antibodies in a mouse E11.5 limb bud. Fold enrichment is presented as a fraction of input. The error bars represent the SD from two technical replicates of a representative experiment. (E) A schematic representation of minimal enhancer sequences with the location of the predicted binding site for TFAP2 (red bar) and LMX1B (green bar) shown.
Rabbit Anti Sox9, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-sox9/product/Millipore
Average 90 stars, based on 1 article reviews
rabbit anti-sox9 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

90
Becton Dickinson mouse anti-e-cadherin
(A) In vitro eTw-5 activity in HEK293T cells following co-transfection with LMX1B or <t>TFAP2</t> (TFAP2α and/or TFAP2γ) expression vectors. Luciferase activity was measured for the eTw-5 sequence and for deleted LMX1B (ΔLMX1B), TFAP2 (ΔTFAP2) or both LMX1B and TFAP2 (ΔLMX1B\ΔTFAP2) binding sites from eTw-5 sequence. (B) In vitro eTw-6 activity in HEK293T cells following co-transfection with LMX1B or TFAP2 (TFAP2α and/or TFAP2γ) expression vectors. Luciferase activity was measured for the eTw-6 sequence and deleted LMX1B (ΔLMX1B), TFAP2 (ΔTFAP2) or both LMX1B and TFAP2 (ΔLMX1B\ΔTFAP2) binding sites from eTw-6 sequence. (C) In vitro eTw-7 activity in HEK293T cells following co-transfection with LMX1B expression vector. Luciferase activity was measured for the eTw-7 sequence and for LMX1B-deleted eTw-7 (ΔLMX1B). Relative luciferase expression results after normalization to Renilla activity are presented and represent the means ± standard deviation of three independent experiments (P-value<0.05, Student’s t-test). (D) ChIP-qPCR analysis of eTw5-7 with anti-TFAP2α antibodies in a mouse E11.5 limb bud. Fold enrichment is presented as a fraction of input. The error bars represent the SD from two technical replicates of a representative experiment. (E) A schematic representation of minimal enhancer sequences with the location of the predicted binding site for TFAP2 (red bar) and LMX1B (green bar) shown.
Mouse Anti E Cadherin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-e-cadherin/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
mouse anti-e-cadherin - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

90
Thermo Fisher mouse anti-zo1
(A) In vitro eTw-5 activity in HEK293T cells following co-transfection with LMX1B or <t>TFAP2</t> (TFAP2α and/or TFAP2γ) expression vectors. Luciferase activity was measured for the eTw-5 sequence and for deleted LMX1B (ΔLMX1B), TFAP2 (ΔTFAP2) or both LMX1B and TFAP2 (ΔLMX1B\ΔTFAP2) binding sites from eTw-5 sequence. (B) In vitro eTw-6 activity in HEK293T cells following co-transfection with LMX1B or TFAP2 (TFAP2α and/or TFAP2γ) expression vectors. Luciferase activity was measured for the eTw-6 sequence and deleted LMX1B (ΔLMX1B), TFAP2 (ΔTFAP2) or both LMX1B and TFAP2 (ΔLMX1B\ΔTFAP2) binding sites from eTw-6 sequence. (C) In vitro eTw-7 activity in HEK293T cells following co-transfection with LMX1B expression vector. Luciferase activity was measured for the eTw-7 sequence and for LMX1B-deleted eTw-7 (ΔLMX1B). Relative luciferase expression results after normalization to Renilla activity are presented and represent the means ± standard deviation of three independent experiments (P-value<0.05, Student’s t-test). (D) ChIP-qPCR analysis of eTw5-7 with anti-TFAP2α antibodies in a mouse E11.5 limb bud. Fold enrichment is presented as a fraction of input. The error bars represent the SD from two technical replicates of a representative experiment. (E) A schematic representation of minimal enhancer sequences with the location of the predicted binding site for TFAP2 (red bar) and LMX1B (green bar) shown.
Mouse Anti Zo1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-zo1/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
mouse anti-zo1 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

90
DIAGENODE DIAGNOSTICS bioruptor
(A) In vitro eTw-5 activity in HEK293T cells following co-transfection with LMX1B or <t>TFAP2</t> (TFAP2α and/or TFAP2γ) expression vectors. Luciferase activity was measured for the eTw-5 sequence and for deleted LMX1B (ΔLMX1B), TFAP2 (ΔTFAP2) or both LMX1B and TFAP2 (ΔLMX1B\ΔTFAP2) binding sites from eTw-5 sequence. (B) In vitro eTw-6 activity in HEK293T cells following co-transfection with LMX1B or TFAP2 (TFAP2α and/or TFAP2γ) expression vectors. Luciferase activity was measured for the eTw-6 sequence and deleted LMX1B (ΔLMX1B), TFAP2 (ΔTFAP2) or both LMX1B and TFAP2 (ΔLMX1B\ΔTFAP2) binding sites from eTw-6 sequence. (C) In vitro eTw-7 activity in HEK293T cells following co-transfection with LMX1B expression vector. Luciferase activity was measured for the eTw-7 sequence and for LMX1B-deleted eTw-7 (ΔLMX1B). Relative luciferase expression results after normalization to Renilla activity are presented and represent the means ± standard deviation of three independent experiments (P-value<0.05, Student’s t-test). (D) ChIP-qPCR analysis of eTw5-7 with anti-TFAP2α antibodies in a mouse E11.5 limb bud. Fold enrichment is presented as a fraction of input. The error bars represent the SD from two technical replicates of a representative experiment. (E) A schematic representation of minimal enhancer sequences with the location of the predicted binding site for TFAP2 (red bar) and LMX1B (green bar) shown.
Bioruptor, supplied by DIAGENODE DIAGNOSTICS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bioruptor/product/DIAGENODE DIAGNOSTICS
Average 90 stars, based on 1 article reviews
bioruptor - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


(A) In vitro eTw-5 activity in HEK293T cells following co-transfection with LMX1B or TFAP2 (TFAP2α and/or TFAP2γ) expression vectors. Luciferase activity was measured for the eTw-5 sequence and for deleted LMX1B (ΔLMX1B), TFAP2 (ΔTFAP2) or both LMX1B and TFAP2 (ΔLMX1B\ΔTFAP2) binding sites from eTw-5 sequence. (B) In vitro eTw-6 activity in HEK293T cells following co-transfection with LMX1B or TFAP2 (TFAP2α and/or TFAP2γ) expression vectors. Luciferase activity was measured for the eTw-6 sequence and deleted LMX1B (ΔLMX1B), TFAP2 (ΔTFAP2) or both LMX1B and TFAP2 (ΔLMX1B\ΔTFAP2) binding sites from eTw-6 sequence. (C) In vitro eTw-7 activity in HEK293T cells following co-transfection with LMX1B expression vector. Luciferase activity was measured for the eTw-7 sequence and for LMX1B-deleted eTw-7 (ΔLMX1B). Relative luciferase expression results after normalization to Renilla activity are presented and represent the means ± standard deviation of three independent experiments (P-value<0.05, Student’s t-test). (D) ChIP-qPCR analysis of eTw5-7 with anti-TFAP2α antibodies in a mouse E11.5 limb bud. Fold enrichment is presented as a fraction of input. The error bars represent the SD from two technical replicates of a representative experiment. (E) A schematic representation of minimal enhancer sequences with the location of the predicted binding site for TFAP2 (red bar) and LMX1B (green bar) shown.

Journal: PLoS Genetics

Article Title: Unraveling the transcriptional regulation of TWIST1 in limb development

doi: 10.1371/journal.pgen.1007738

Figure Lengend Snippet: (A) In vitro eTw-5 activity in HEK293T cells following co-transfection with LMX1B or TFAP2 (TFAP2α and/or TFAP2γ) expression vectors. Luciferase activity was measured for the eTw-5 sequence and for deleted LMX1B (ΔLMX1B), TFAP2 (ΔTFAP2) or both LMX1B and TFAP2 (ΔLMX1B\ΔTFAP2) binding sites from eTw-5 sequence. (B) In vitro eTw-6 activity in HEK293T cells following co-transfection with LMX1B or TFAP2 (TFAP2α and/or TFAP2γ) expression vectors. Luciferase activity was measured for the eTw-6 sequence and deleted LMX1B (ΔLMX1B), TFAP2 (ΔTFAP2) or both LMX1B and TFAP2 (ΔLMX1B\ΔTFAP2) binding sites from eTw-6 sequence. (C) In vitro eTw-7 activity in HEK293T cells following co-transfection with LMX1B expression vector. Luciferase activity was measured for the eTw-7 sequence and for LMX1B-deleted eTw-7 (ΔLMX1B). Relative luciferase expression results after normalization to Renilla activity are presented and represent the means ± standard deviation of three independent experiments (P-value<0.05, Student’s t-test). (D) ChIP-qPCR analysis of eTw5-7 with anti-TFAP2α antibodies in a mouse E11.5 limb bud. Fold enrichment is presented as a fraction of input. The error bars represent the SD from two technical replicates of a representative experiment. (E) A schematic representation of minimal enhancer sequences with the location of the predicted binding site for TFAP2 (red bar) and LMX1B (green bar) shown.

Article Snippet: Immunoprecipitation was performed using 5 mg of anti-Tfap2α antibodies (sc-12726, Santa-Cruz Biotechnology).

Techniques: In Vitro, Activity Assay, Cotransfection, Expressing, Luciferase, Sequencing, Binding Assay, Plasmid Preparation, Standard Deviation, ChIP-qPCR