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Image Search Results
Journal: PLoS Genetics
Article Title: Unraveling the transcriptional regulation of TWIST1 in limb development
doi: 10.1371/journal.pgen.1007738
Figure Lengend Snippet: (A) In vitro eTw-5 activity in HEK293T cells following co-transfection with LMX1B or TFAP2 (TFAP2α and/or TFAP2γ) expression vectors. Luciferase activity was measured for the eTw-5 sequence and for deleted LMX1B (ΔLMX1B), TFAP2 (ΔTFAP2) or both LMX1B and TFAP2 (ΔLMX1B\ΔTFAP2) binding sites from eTw-5 sequence. (B) In vitro eTw-6 activity in HEK293T cells following co-transfection with LMX1B or TFAP2 (TFAP2α and/or TFAP2γ) expression vectors. Luciferase activity was measured for the eTw-6 sequence and deleted LMX1B (ΔLMX1B), TFAP2 (ΔTFAP2) or both LMX1B and TFAP2 (ΔLMX1B\ΔTFAP2) binding sites from eTw-6 sequence. (C) In vitro eTw-7 activity in HEK293T cells following co-transfection with LMX1B expression vector. Luciferase activity was measured for the eTw-7 sequence and for LMX1B-deleted eTw-7 (ΔLMX1B). Relative luciferase expression results after normalization to Renilla activity are presented and represent the means ± standard deviation of three independent experiments (P-value<0.05, Student’s t-test). (D) ChIP-qPCR analysis of eTw5-7 with anti-TFAP2α antibodies in a mouse E11.5 limb bud. Fold enrichment is presented as a fraction of input. The error bars represent the SD from two technical replicates of a representative experiment. (E) A schematic representation of minimal enhancer sequences with the location of the predicted binding site for TFAP2 (red bar) and LMX1B (green bar) shown.
Article Snippet: Immunoprecipitation was performed using 5 mg of
Techniques: In Vitro, Activity Assay, Cotransfection, Expressing, Luciferase, Sequencing, Binding Assay, Plasmid Preparation, Standard Deviation, ChIP-qPCR